Abstract: Objective To explore the protective mechanism of reduced enteral feeding on preterm infants with transfusionassociated necrotizing enterocolitis through the reduced enteral feeding intervention in preterm infants with transfusion-associated necrotizing enterocolitis. Method A total of 100 premature infants with transfusion-associated necrotizing enterocolitis treated in our hospital between January 2018 and December 2019 were enrolled as the research objects. The random number table method was used to devide the participants into a control group and a test group with 50 cases in each group. The control group was fed as normal, and the test group was fed reduced amounts. The plasma TNF-α and IL-6 concentrations before and after blood transfusion and the occurrences of TRNEC within 48 hours of blood transfusion were compared between the two groups of premature infants. Result There was no statistically significant difference in the concentrations of TNF-α and IL-6 between the two groups(P> 0. 05). At 6 and 12 hour after blood transfusion, the concentrations of TNF-α and IL-6 in the test group were significantly lower than those in the control group(P<0.05). At 6 and 12 hour after blood transfusion, the concentrations of TNF-α and IL-6 in the test group were significantly higher than those before blood transfusion(P< 0. 05). Within 48 hours of blood transfusion, the proportion of TRNEC in the test group and the proportion of symptoms such as vomiting, abdominal distension, and bloody stool were lower than those in the control group(P<0. 05). Conclusion Reduced enteral feeding could effectively reduce the concentrations of TNF-α and IL-6 after blood transfusion after premature delivery, reduce the incidence of TRNEC, and help prevent transfusion-related necrotizing enterocolitis in preterm infants.