Abstract: Objective To analyze and study the clinical value of screening for irregular antibodies in pregnant and non-pregnant women receiving blood transfusions.Methods This study was conducted in 120 women who were admitted to our hospital from March 2017 to March 2019. They were divided into non-pregnant women (n = 70 cases) and pregnant women (n = 50 cases) depending on whether they were pregnant. The irregular antibody screening and the specific distribution of irregular antibody screening in blood recipients were carried out,and the positive rate of irregular antibody screening in pregnant and non-pregnant women was analyzed.Results The total screening rate of irregular antibodies in 120 recipients was 29. 16%,of which anti-M occupied 5. 83%,anti-D occupied 5. 00%,anti-E occupied 4. 16%,anti-C occupied 3. 33%,Aanti-PI occupation ratio 2. 50%,anti-Leb occupancy ratio 1. 67%,anti-Lea occupation ratio 1. 67%,anti-H occupation ratio 0. 83%,anti-H occupation ratio 0. 83%,anti-Fyb occupation ratio 0. 83%,-N occupied 0. 83%,anti-JKa occupied 0. 83%,and non-specific antibody occupied 1. 67%. The positive rate of irregular antibody screening in non-pregnant women was 21. 42%,which was lower than that in pregnant women (40. 00%),and the difference was statistically significant (P< 0. 05).Conclusion Before carrying out blood transfusion for female patients,especially pregnant women,it is necessary to carry out screening of irregular antibodies in recipients,in order to ensure the overall blood transfusion safety.