Abstract: Objective To investigate the effects of sperm DNA damage on embryo quality and clinic outcome of IVF-ET. Method The data of 125 infertile couples who were treated with conventional in vitro fertilization(IVF) and intracytoplasmic sperm microinjection(ICSI) in the reproductive center of our hospital were analyzed retrospectively. The participants were divided into normal group, low injury group, and high injury group according to sperm DNA fragment index(DFI) < 15%, 15%-30%, and >30%, respectively. The normal fertilization rates, normal cleavage rates, high-quality embryo rates, blastocyst formation rates, and clinical pregnancy rates were compared among different groups treated with IVF-ET. Result In the cycle of IVF or ICSI treatment,there were no significant differences in the effects of DNA damage on normal fertilization rate, normal cleavage rate, high-quality embryo rate, and clinical pregnancy rate in the DFI < 30% groups(P> 0. 05). In the DFI > 15% groups, the blastocyst formation rates decreased significantly(P< 0. 05). Conclusion During fresh embryo transfer, sperm DNA damage had no significant effects on IVF-ET outcome but affected blastocyst formation rate in the patients with DFI >15%.