Abstract: Objective To explore the effect of multidisciplinary cooperative health education in prevention and treatment of children's attention deficit hyperactivity disorder. Method By the case-control study, children came to Guangdong Province Hospital for Women and Children Health care from January 2018 to May 2019,and were diagnosed with ADHD,were selected and divided into experimental group and control group. The control group was conducted with conventional treatment, while multi-disciplinary collaborative health education was added to the experimental group on the basis of conventional treatment. The Conners Parental Scale was used to investigate the scores of the two groups of children before and after treatment, and to compare the treatment effects. Result Through comparative analysis, it was found that the children in the experimental group had an average score of 44. 38 at diagnosis and an average score of 28. 45 after intervention. The control group had an average score of 45. 41 at diagnosis and an average score of 32. 24 after intervention. Therefore, the experimental group had better intervention effects than those in the control group.Conclusion Multidisciplinary cooperative health education can improve the prevention and treatment effect of children with attention deficit hyperactivity disorder.