Abstract: Objective To study the impact of perinatal group B streptococcus(GBS) and chlamydia reproductive tract infection(RTI) on pregnancy outcomes. Methods 294 pregnant women and their newborns who were discharged from Xuchang hospital from September 2018 to August 2020 were recruited as study subjects. All pregnant women underwent vaginal smear examination. They were divided into 4 groups based on the results: group A(116 cases,no infection),group B(56 cases,simple GBS infection),group C(68 cases,simple Chlamydia infection),and group D(54 cases,mixed infection of GBS and Chlamydia). The incidence of perinatal complications and neonatal abnormalities were compared within four groups. Results The incidence of total maternal and infant complications were group D,group B,group C and group A in turn from higher to lower level. The rate of cesarean section in group D was higher than that in other three groups(P< 0. 05),and preterm birth,meconium-stained amniotic fluid and premature rupture of membranes in group D were higher than those in group A(P< 0. 05); The total incidence of neonatal abnormalities was group D,group B,group C,and group A in turn from higher to lower level,the difference was statistically significant(P< 0. 05). Among these,neonatal asphyxia,fetal distress,neonatal infection in group D were higher than those in group A,neonatal infection in group D was higher than that in group C,the difference was statistically significant(P< 0. 05). Conclusion Mixed infection of GBS and chlamydia will increase the incidence of perinatal maternal-neonatal complications and neonatal abnormalities. It is of clinical importance to detect GBS and chlamydia infection for perinatal women.