Abstract: Objective To investigate the pathogens that can easily cause fetal infection and abnormal development among women during pregnancy preparation including toxoplasma(TOX), others(O), rubella virus(RV), cytomegalovirus(CMV), and herpes simplex virus(HSV)(TORCH), and to provide data support for women and children's health care in Shihezi City, Xinjiang Uygur Autonomous Region.Methods A total of 4 721 women who underwent pre-pregnancy health examination in Shihezi City, Xinjiang Uygur Autonomous Region from 2017 to 2020 were recruited. RV, TOX and CMV were detected by conventional colloid gold method.hepatitis B virus(HBV), treponema pallidum(TP) and human immunodeficiency virus(HIV) were detected by enzyme-linked immunosorbent assay(ELISA). They were divided into different groups according to age, season and year for comparison.Results The positive rates of specific antibodies of HIV, TP and hepatitis B surface antigen(HBsAg) were 0. 02%, 0. 30% and 4. 43% in4 721 cases, respectively. The positive rate of CMV-IgM(3. 00%) was higher than that of TOX-IgM(1. 91%). RV-IgM positive rate(93. 96%) ranked the highest. The positive rates of HBs Ag, RV-IgG and CMV-IgG were different in various age groups(P< 0. 05).Except for HBs Ag, HIV, TP and RV-IgG, the detection rates of other pathogens were different in various seasons(all P< 0. 05).Except for HBs Ag, HIV and TP, the positive rates of other pathogens were different in various years(all P< 0. 05).Conclusion People are susceptible to TORCH in different ages and seasons in Shihezi City, Xinjiang Uygur Autonomous Region. Relevant measures should be taken to strengthen pre-pregnancy health examination in order to to prevent from congenital TORCH infection and improve the quality of newborns.