Abstract: Objective To investigate the rate and factors associated with nutrient supplement use among pregnant women in Tongzhou District, Beijing so as to provide evidence for guiding rational use of nutrient supplement for pregnant women. Methods Totally 1181 pregnant women who received routine prenatal care at 2 public hospitals in Tongzhou District were recruited. Rate and factors associated with nutrient supplement taking behavior among pregnant women were investigated through face-to-face questionnaire survey. Results The percentage of folic acid, complex folic acid, calcium and iron intake among pregnant women were 55. 3%,39. 3% and 21. 7%, respectively. Gestational week, education background, annual family income were factors associated with nutrient supplement intake. The percentage of folic acid and complex folic acid(58. 3%), vitamin D(16. 4%), calcium(43. 3%), iron(24. 9%) and multivitamin(26. 3%) intake were higher among women at late pregnancy(P< 0. 05). The percentage of folic acid and complex folic acid(62. 8%), B group vitamins(15. 5%) and vitamin D(19. 7%) intake were higher among those with education background higher than university degree(P< 0. 05). The percentage of B group vitamins(15. 2%) and calcium(44. 2%)intake were the highest among pregnant women whose annual family income was over 50 000(P< 0. 05). Logistic regression analysis showed that pregnancy experience and high education level were factors promoting the use of folic acid and complex folic acid(P<0. 05). Conclusion Nutrient supplements intake is popular among pregnant women in Tongzhou District, Beijing. But nutrition education should be carried out based on different characteristics of people to help pregnant women choose nutrient supplements scientifically and rationally.