Abstract: Objective To describe different ways of pregnancy health care knowledge accessing and explore the factors associated with it.Methods A self-designed questionnaire was used to investigate general information and ways of pregnancy health care knowledge access among pregnant women and mothers with infants aged 0-1 in 3 county-level Maternal and Child Health Care Institutions at Guangxi, Hunan and Hebei provinces. Logistic regression analysis was used to explore factors associated with various ways of acquiring pregnancy health care knowledge.Results A total of 1 718 questionnaires were distributed and 1 673 valid questionnaires were received, including 400 pregnant women and 1 273 mothers with infant. The top way of accessing pregnancy health care information was consulting with doctors, accounting for 70.6%(1 181/1 673). The second was through the internet, accounting for 42.6%(713/1 673).Logistic regression analysis showed that the people in Hebei and Hunan were significantly more likely to use new media and traditional media than those in Guangxi(P<0.05). Compared with early pregnancy women, middle pregnancy women(OR=5.67, 95%CI:1.58-36.37) were more likely to consult with medical staff, while women 6-<9 months postpartum(OR=0.64, 95%CI:0.43-0.95) were less likely to use. Subjects who had pre-pregnancy examinations were less likely to use traditional media(OR=0.80, 95%CI:0.65-0.99). Subjects with older age(OR=0.97, 95%CI:0.95-0.99) were less likely to use new media. Subjects with high school education level or above(OR=1.81, 95%CI:1.44-2.28), non-peasant occupation(OR=1.41, 95%CI:1.11-1.79) and who had undergone premarital examination(OR=1.37, 95%CI:1.11-1.70) were more likely to adopt new media.Conclusion Internet and new media should be used rationally to help pregnant women acquire knowledge of pregnancy health care. Health education on pregnancy care should be carried out systematically and pertinently, and pregnant women with low education background, low income, being older, and not undergoing premarital examination should be on paid more attention, so as to improve their awareness of pregnancy health care comprehensively.