Abstract: Objective To investigate the current situation of visual acuity and refraction among kindergarten children in Binhu District, Wuxi, so as to provide evidence for eye care service and preventive strategies of eye diseases.Methods A cross-sectional survey was conducted in Binhu District. A total of 13 017 children aged 4-7 years old in all kindergartens were recruited. Their naked-eye vision was investigated through standard logarithmic visual acuity chart and refractive parameters were measured by automatic computer optometry. Multivariate logistic regression analysis was used to explore factors associated with myopia.Results A total of 7 425 children(57.79%) were found poor vision, 3 589 children(27.93%) were found refractive abnormalities, and 1 836 children(14.29%) were found screening myopia. Among them, the rates of poor vision and screening myopia among children aged 5-<6 were higher than those of children aged 4-<5 and 6-7, and the differences were statistically significant(χ²=242.707, 25.391, all P<0.05). The rates of poor vision in private and rural kindergartens were higher than those in public and urban kindergartens, respectively(χ²=4.658, 46.911, all P<0.05). There were significant differences in the rates of poor vision and refractive abnormalities among kindergarten children of different education levels(χ²=60.571,46.067, all P<0.05). Logistic regression analysis showed that age, the nature of kindergarten and education level were the factors associated with children's screening myopia rate. Conclusion The rate of visual impairment is relatively high among kindergarten children in Binhu District, Wuxi. It is suggested that health education of eye care and visual health examination should be strengthened, and the children in rural, private and low education level kindergartens should be emphasized.