Objective To investigate knowledge and practices of Corona Virus Disease 2019（COVID-19） prevention among pregnant women, so as to provide evidence for further health education and promotion.

Methods Convenient sampling was used to recruit 1 014 pregnant women as study subjects from February 27^th to March 6^th 2020. Self-designed internet-based online questionnaire survey was conducted through Healthy Family App. The knowledge and practices of COVID-19 as well as the information accessing was analyzed.

Results The overall awareness rate of COVID-19 prevention was 87.7%. And 94.8% of the respondents indicated that the virus could be transmitted from human to human, and 95.3% agreed that keeping windows open and ventilated was helpful for COVID-19 prevention. However, some of them considered saltwater mouthwash （24.8%） and garlic（15.8%） were effective measures to prevent COVID-19. The awareness rate of COVID-19 prevention among primipara（89.9%） was higher than that among parturient （83.8%）, and the difference was statistically significant （χ² = 47.971,P < 0.05）. Pregnant women mainly accessed information of COVID-19 prevention through medical staff （59.1%）, internet and social media platform （47.0%）, radio and/or television（39.1%）. 36.0% respondents received information through three or more ways. The main preventive measures taken by pregnant women were wearing face masks outside （92.8%）, washing hands frequently （88.8%） and staying indoor most of the time （88.0%）. The proportion of primipara taking these three measures concurrently was higher than that of primipara （χ² = 6.283, P = 0.012）.

Conclusion Pregnant women have good understandings and protection ability of COVID-19 prevention. However, misunderstandings still exist. It’s necessary to carry out advocacy campaign through a variety of channels, especially to give full play to the role of medical personnel, as well as to strengthen the advocacy and management of parturient.