LncRNA-HOTAIR靶向miR-148a-3p调控乳腺癌细胞增殖、侵袭
LncRNA-HOTAIR regulates the proliferation and invasion of breast cancer cells by targeting miR-148a-3p
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摘要:目的 探讨长链非编码RNA（long non-coding RNA, LncRNA）HOX转录反义RNA（HOX transcript antisense RNA, HOTAIR）调控miR-148a-3p对乳腺癌细胞增殖、侵袭的影响。方法 通过转染实验, 将乳腺癌细胞T47D分为对照组、HOTAIR过表达组以及HOTAIR表达抑制组。采用实时荧光定量聚合酶链反应（quantitative real-time polymerase chain reaction, qRT-PCR）方法检测3组细胞中HOTAIR与miR-148a-3p的表达情况；Cell Counting Kit-8（CCK-8）法及Transwell小室法检测各组转染细胞增殖、侵袭情况；双荧光素酶法检测HOTAIR与miR-148a-3p的靶向结合情况。结果 qRT-PCR显示T47D细胞中, HOTAIR过表达与抑制效果显著（P < 0.05）。与抑制组比较, 过表达组T47D细胞中miR-148a-3p表达水平明显下降（P < 0.05）, 细胞增殖及侵袭能力均升高（P < 0.05）。野生型HOTAIR序列与miR-148a-3p模拟物共转染细胞的荧光素酶活性降低（P < 0.05）。结论 LncRNA-HOTAIR可通过miR-148a-3p影响T47D细胞的增殖、侵袭能力。Abstract:Objective To investigate the effects of long non-coding RNA(LncRNA) HOX transcript antisense RNA(HOTAIR) on the proliferation and invasion of breast cancer cells by targeting miR-148a-3p.Methods T47D cell line were divided into control group, HOTAIR overexpression group and HOTAIR inhibition group through transfection experiment. Quantitative real-time polymerase chain reaction(qRT-PCR) was used to detect the expression of HOTAIR and miR-148a-3p in all 3 groups. Cell Counting Kit-8(CCK-8) and Transwell chamber method were used to detect the proliferation and invasion of transfected T47D cells. The targeted binding of HOTAIR to miR-148a-3p was detected by dual luciferase method.Results The qRT-PCR showed that overexpression and inhibition of HOTAIR were significant(P < 0.05). Compared with the inhibition group, the expression level of miR-148a-3p in the overexpression group was significantly decreased(P < 0.05), and cell proliferation and invasion ability were significantly increased(P < 0.05). The luciferase activity of cells co-transfected with wild-type HOTAIR sequence and miR-148a-3p mimics was decreased (P < 0.05).Conclusion LncRNA-HTOAIR may affect the proliferating and invading ability of T47D cells through miR-148a-3p.
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