Abstract: Objective To investigate the expression of Survivin and Ki-67 protein in uterine fibroids and the relationship between them and the occurrence and development of myoma.Methods 90 cases of uterine leiomyoma (myoma group) and 30 cases of normal myometrial tissues (normal group) in our hospital obstetrics and Gynecology were collected after surgery. The collection time was from April 2016 to May 2017, and immunohistochemistry was used. The positive rate of Survivin and Ki-67 protein in the two groups were detected by staining. The relationship between the Survivin and Ki-67 protein and the menopausal status of the uterine fibroids was discussed.Results The positive expression rate of Survivin protein in patients with leiomyoma was 81. 11% and it was significantly higher than that in the normal group (13. 33%) (P<0. 05). The positive expression rate of Ki-67 proteinin myoma group was 10%.It was significantly lower than that in the normal group (6. 67%) (P> 0. 05). The positive expression rates of leiomyoma tissue Survivin protein among patients with uterine fibroids diameter over 5 cm and multiple uterine myomas were significantly higher than those among patient with uterine fibroids diameter < 5 cm and single uterine myoma (P<0. 05). There was no statistical difference in the positive rate expression of leiomyoma tissue Survivin protein between postmenopausal and premenopausal patients (P> 0. 05). There was significantly statistical difference in the positive expression rate of Ki-67 protein between patients with different fibroids diameter, with or without menopause, and multiple and single uterine myoma (P> 0. 05).Conclusion Survivin protein is highly expressed in uterine fibroids. However, the change of Ki-67 protein expression is not obvious in uterine fibroids. The positive expression of Survivin protein has a certain relationship with the occurrence and development of uterine fibroids.