Abstract: Objective To detect the expression and clinical significance of Olfactomedin 4 (OLFM4) and Quinome Oxidoreductase-1 (NQO1) in cervical carcinoma and to explore their relationships.Methods Immunohistochemistry SP was used to evaluate the expression of OLFM4 and NQO1 in 40 cervical carcinoma tissues, 20 normal cervical tissues, 20 samples from adjacent tissues.Results In cervical carcinoma tissues, the positive expression of OLFM4 and NQO1 were 92.5% (37/40) and 77.5% (31/40), respectively. In normal cervical tissues, the positive rates were 20% (4/20) and 30% (6/40), respectively. In the adjacent tissues, the positive rates were 25% (5/20) and 40% (8/20), respectively. They were both significantly lower than those in normal cervical carcinoma tissures. Meanwhile, the expression level of OLFM4 was correlated with histological differentiation, but was not correlated with age, clinical stage, tumor size, invasion depth or lymph node metastasis. The expression of NQO1 was correlated with differentiation and lymph node metastasis, but was not correlated with age, clinical grade, tumor size, invasion depth. A positive correlation between the expression of OLFM4 and NQO1 was observed (r=0.355, P< 0.05).Conclusions In cervical carcinoma tissues, the expression of OLFM4 and NQO1 were up-regulated, which were tightly related with the genesis and development of cervical carcinoma. And they were considered as markers for estimating the prognosis of cervical carcinoma in some extent.