Abstract: Objective To understand the basic situation,source and demand of reproductive health knowledge of junior high school students in Futian District of Shenzhen city,in order to provide base for adolescent reproductive health education.Methods Random cluster sampling method was used and 1029 junior high school students in Futian District of Shenzhen city were selected for the questionnaire survey.Results Among the 1029 students,most of them contacted sex education first in junior high school,this matched the time that they were supposed to be educated with reproductive knowledge. Students had higher awareness of basic reproductive health knowledge,66.2% of them know about the common reproductive health questions. The awareness rate of menstruation,seminal emission and sexual impulse was 83.1%,76.4% and 82.8% respectively. The main sources of sex knowledge were schools,internet films,classmates or friends. Only 36% of the girls and 16.8% of the boys got sexual knowledge from their parents. The students expect the sexual education methods were lectures,videos,brochures and human body parts explaining. The demand of sexual education focus on sexual psychology, sexual physiological knowledge, sexual responsibilities and options.Conclusion Junior high school students in Futian District of Shenzhen had high awareness of reproductive health knowledge,but sexual psychology education and demand were obvious. The ways of sex knowledge is various and family education obtaining is low. We should focus on adolescent psychological guidance and education; give full attention to family education. School,hospital and family resources should be combined,in order to seek for the best mode for adolescent reproductive health.