Abstract: Objective To investigate the syphilis testing situation among partners of syphilis infected pregnant women in Guangdong province,and to analysis the associated factors about syphilis testing,in order to provide a reference of continuous improvement for Project of Syphilis Prevention of Mother-to-child Transmission (PMTCT) in Guangdong.Methods All data were collected from national integrated PMTCT surveillance system.Chi-square test and multinomial Logistic regression were employed to analysis the influence factors about the syphilis testing and infection status among partners of syphilis infected pregnant women.Results A total of 1476 cases of syphilis infected pregnant women and their sexual partners were involved in this study.Only 25.3% of sexual partners' syphilis infection status had been diagnosed,and their syphilis infection rate was 24.7%.Compared with the group of 21-35 age,the partners of pregnant women infected and elder than 36 age were less likely to have a clear diagnosis (adjusted OR=0.61,95%CI:0.43-0.88).Compared with the pregnant women infected unknown education degree,partners of pregnant women with a degree of education in the university or higher were more likely have their status diagnosed (adjusted OR=5.97,95%CI:2.51-14.19);Partners of pregnant women with infection history were more likely to had a clear diagnosis (adjusted OR=1.92,95%CI:1.47-2.51).Compared with the first trimester,the later periods the pregnant women diagnosed,the less possibility their partner could be recognized,and the pregnant women diagnose in postpartum period or other stages had the least likely with a identified partner (adjusted OR=0.36,95%CI:0.21-0.63).Compared with sexually transmitted,pregnant women infected unknown or others,their partners had less a syphilis infected partner (adjusted OR=0.25,95%CI:0.16-0.42).Conclusion The syphilis testing rate among partners of syphilis infected pregnant women in Guangdong was very low.It should be strengthened propaganda and education on the elder and lower education level pregnant women,to promoting active syphilis screening and treatment among pregnant women and their sexual partner,and to avoid the harm for infant of the pregnant women l repeated infection.