Abstract: Objective To explore the changes of blood glucose and blood lipid items of healthy pregnant women in Baoan District of Shenzhen, and to establish the reference interval of normal pregnant women.Methods 1200 cases healthy pregnant women from 4 to 40 pregnancy weeks were recruited by the Baoan District maternal and child health care hospital from November 2017 to August 2018. They were grouped according to their age and gestational weeks, and 150 cases were in each group. The blood glucose and blood lipid items were detected to establish the reference range of blood glucose and blood lipid items of healthy pregnant women in Baoan District of Shenzhen.Results Compared with the 19-32 years old group, the serum levels of FPG, TG and Apo B increased significantly, and the levels of HDL and Apo A significantly decreased in the 33-45 years old group (P < 0. 05). There were no significant differences in TC and LDL levels between different age groups (P> 0. 05). There were significant differences in blood sugar and blood lipid indexes between different gestational weeks (P < 0. 05). Among them, the level of serum FPG was the highest in the early pregnancy, the lowest in second trimester of pregnancy, and then increased with the prolongation of the gestational weeks. The levels of serum TC, TG, Apo A and Apo B increased with the prolongation of gestational weeks. The level of serum HDL increased with gestational age, reached the highest level in the third trimester of pregnancy and decreased slightly before delivery. The serum LDL level did not show a correlation with gestational age.Conclusion There are differences in blood sugar and blood lipid among healthy pregnant women with different ages and gestational weeks. The reference interval of blood glucose and blood lipid indexes of healthy pregnant women with different age and gestational weeks was initially established.