Abstract: Objective To explore the willingness of breast cancer screening by mammography examination and associated factors for breast cancer screening among women over 40 years and without breast diseases in Suzhou. Method A cross-sectional study was conducted among women aged over 40 years and without breast diseases using a self-administered questionnaire. Health belief model was used to analyze the influencing factors for the screening willingness. Result 644 women were included in the analysis. Among them, 65. 84% had intention to participate in free breast cancer screening, 65. 84% were willing to conduct breast cancer screening at their own expense, and 47. 83% were willing to accepted breast mammography examination in the next year. Multivariate Logistic analyses showed that younger, married, better education, higher score in perceived susceptibility, family members or friends suffering from breast cancer and accepted clinical breast examination previously were more likely to accept mammography screening in the next year. Women with higher score of perceived barriers were less likely to accept mammography (P<0. 05). There was statistically significant difference between women with and without screening willingness. However, there was no statistically significant difference in registered residence, occupation, scores of perceived severity, perceived benefits and self-efficacy between women with and without screening willingness.Conclusion Women without breast cancer aged over 40 years in Suzhou have the willingness to participate in breast cancer screening. However, they are lack of willingness to implement in the near future. Age, education level, marital status, family members or friends suffering from breast cancer and accepted clinical breast examination previously play important roles in breast cancer mammography willingness. In order to improve their willingness of breast cancer screening and the compliance behavior, it is important to strengthen women's knowledge of breast cancer, increase perceived susceptibility and decrease perceived barriers