Abstract: Objective To explore the fertility desire and influence factor of two children among female teachers from university.Methods Random sampling method was adopted to carry out the survey on 253 female university teachers among 6 universities/colleges in Fuzhou.Results Among the total females, 52. 6% of them did not have the fertility desire of two children and 68. 4% of them did not have gender preference, only about 25% of them tend to boy. The teachers showed different fertility desire in the score of two children in different educational background, professional title and whether couples from one-child family, and the differences were statistically significant (P< 0. 05). The first influencing factor of fertility desire in university teachers was interval time of the two children, and the other influencing factors were age and their attitude toward two-child policy.Conclusion There was not strong desire for two children among female university teachers, and there were no intergenerational transmission and sex preference.