Abstract: Objective To analyze and explore the clinical value of combined detection of serum SCC-Ag, CEA and HE4 in the diagnosis of cervical cancer.Methods During 2011-2015, total 180 cases were recruited in in our hospital. Among them, 60 cases were diagnosed with cervical cancer (cervical cancer group), 60 cases were diagnosed with cervical intraepithelial neoplasia (CIN group), and 60 were healthy women (control group). All of the cases were tested the serum levels of SCC-Ag, CEA and HE4. The clinical value of each index or combined indexes in diagnosis of cervical cancer was evaluated.Results The serum levels of SCC-Ag, CEA and HE4 of cervical cancer group were significantly higher than that of CIN group and control group. The difference was statistically significant (P<0.05). The serum levels of SCC-Ag, CEA and HE4 of CIN group were significantly higher than that of control group (P<0.05). The sensitivity of serum SCC-Ag in differential diagnosis of cervical cancer group was 31.67% and the specificity was 66.67%. The sensitivity of serum CEA in differential diagnosis of cervical cancer group was 35% and the specificity was 63.33%. The sensitivity of serum HE4 in differential diagnosis of cervical cancer group was 38.33% and the specificity was56.67%; The sensitivity of combination of serum SCC-Ag, CEA and HE4 tests in diagnosis of cervical cancer was 86.67%, the specificity was 76.67%, the misdiagnosis rate was 13.33%, and the misdiagnosis rate was 23.33%.Conclusion The combination of serum SCC-Ag, CEA and HE4 tests can significantly improve the sensitivity and specificity of cervical cancer diagnosis.