Abstract: Objective To observe the changes of the serum IL-10, IL-5, IL-4 and the levels of immune factors of cervical intraepithelial neoplasms (CIN) patients, and to analyze their clinical significances.Methods A total of 80 CIN patients treated in our hospital from March 2016 to March 2017 were selected as the observation group, and 80 healthy women who underwent physical examination in our hospital were selected as the control group. Observe The differences in serum IL-10, IL-5, IL-4 and the levels of immune factors between the observation group and the control group, and among the different clinical stages of CIN patients were compared. And, the difference in HPV virus load among different clinical stages of CIN patients was compare. The correlations between the levels of IL-10, IL-5, IL-4 sand HPV viral load of volume and clinical stages of CIN patients were analyzed.Results The levels of IL-10, IL-5 and IL-4 of the observation group were higher than those of the control group, and the levels of IFN-γ and TNF-α were lower than those of the control group. The HPV viral load of the observation group was significantly higher than that in the control group (t=-30.885, P< 0.001). The levels of IL-10, IL-5 and IL-4 of patients with CIN II and III were higher than those of stage CIN I, and the levels of IFN-gamma and TNF-alpha were lower than those of CIN stageⅠ. The HPV viral load of patients with CIN II and III was significantly higher than that of stage CIN I patients (t=-6.541, P< 0.001). The HPV viral load was positively correlated with the levels of IL-10, IL-5, IL-4 and clinical stages of CIN patients, and it was negatively correlated with the levels of IFN-γ and TNF-α.Conclusion The levels of serum IL-10, IL-5, IL-4 of CIN patients were higher. The levels of IFN-γand TNF-α of CIN patients were lower. The levels of serum IL-10, IL-5, IL-4, IFN-γ and TNF-α are closely related with the clinical stage and the viral load of CIN patients.