Abstract: Objective To explore the clinical effect of different dose of vitamin D on preventing premature rickets and its'nursing strategy.Methods From Jan 2014 to Jan 2016,85 premature infants who accepted cured in Pingshan District People's Hospital were selected as the study objects,and they were divided into low dose group (400 IU/d) and high dose group (800 IU/d) according to the dose of vitamin D.The incidence of rickets,related biochemical indexes before and after treating,the changes of the effective catch-up growth rate and adverse reaction between the two groups were compared.Results The incidence of rickets in high dose group was 5.6%,while the low group was 18.2%,and the difference was statistically significant (P<0.05);In high dose group,the content of Calcium (3.86 ±1.04mmol/L),effective catch-up growth rate (94.44%),25(OH) D (63.5 ±4.24 ng/L),bone Calcium (19.32 ±1.23μg/L) and bone alkaline phosphatase (291.4 ±56.3 U/L) were all higher than those in low dose group (P<0.05);while there was no statistical difference between the two groups in adverse reactions (P>0.05).Conclusion High dose vitamin D with relevant nursing strategy is more conducive to decrease the rate of premature rickets and without increasing the incidence of adverse reactions,it is worthy of clinical application.