Abstract: Objective To investigate emotional and behavioral problems among 3 to 6 years old preschool children in Yangzhou, to examine whether screen time are related to both emotional and behavioral problems, and to provide the evidences for developing related intervention measures.Methods We selected 2531 children from 7 kindergartens in Yangzhou urban area with clustering sampling and surveyed them with a questionnaire and physical examination. Odds ratio was assessed by multinomial logistic regression.Results In this study, the detection rate of preschool children's emotional and behavioral problems was 6. 4%. Children's age, gender, parents' age, parents' education level and screen time were the influencing factors of the children's emotion and behaviors.After controlling children's family environment, multivariate logistic regression analysis showed that the risk of emotional and behavioral problem in children with screen time > 2 hours per day for one week and from Monday to Friday per day was higher than the ones with less screen time, the ORs were 1. 535 (95% CI:1. 092-2. 163) and 1. 481 (95% CI:1. 016-2. 158), respectively.Conclusion There was a negative impact of longer screen time on emotional and behavioral problems of preschool aged children.