摘要:
目的 探究卡介苗(BCG)干预对IL-4诱导的巨噬细胞炎症细胞因子(IL-12、IL-6、IL-10、MRC-1)和粘附分子(CCL-2)表达的影响。方法 应用PMA (10 ng/m L)与IL-4(10 ng/m L)刺激巨噬细胞,使其向M2转型,并将溶于DMSO的0 mg/L、20 mg/L、50 mg/L及100 mg/L的BCG作用24 h,应用PCR的方法测定各组中IL-12、IL-6、IL-10、MRC-1及CCL-2 mRNA的表达。结果 IL-4刺激后IL-12、IL-6、IL-10、MRC-1及CCL-2 mRNA含量均有显著升高(P<0.01),而给予不同浓度的BCG后,IL-12、IL-6、IL-10、MRC-1及CCL-2 mRNA含量均有显著下降(P<0.01),100 mg/L的BCG作用最为明显。结论 卡介苗能够抑制IL-4刺激的巨噬细胞炎症细胞因子和粘附分子的表达。
Abstract:
Objective To explore the influence of Bacille Calmette-Guerin (BCG) vaccine intervention on the expression of macrophage inflammatory cytokines and adhesion molecules in IL-4 induction.Methods Macrophages were stimulated with PMA (10 ng/ml) and IL-4 (10 ng/ml), and transformed into M2. Then they were dissolved at DMSO 0 mg/L, 20 mg/L, 50 mg/L and 100 mg/L BCG for 24 hours. The expressions of IL-12, IL-6, IL-10, MRC-1 and CCL-2 mRNA in each group were determined by PCR methods.Results After IL-4 stimulation, the contents of IL-12, IL-6, IL-10, MRC-1 and CCL-2 mRNA were increased significantly (P<0.01). After dissolved in BCG, the contents of IL-12, IL-6, IL-10, MRC-1 and CCL-2 mRNA were decreased significantly (P<0.01). It was more significant after dissolved at 100 mg/L BCG.Conclusion The BCG vaccine can inhibit the expression of the macrophage inflammatory cytokines (IL-12, IL-6, IL-10, MRC-1) and Adhesion molecule (CCL-2) which were stimulated by IL-4.
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