Abstract: Objective To analyze the mental health and related factors of women during pregnancy in Puning City.Methods A total of 467 pregnant women admitted to Gynecology and Obstetrics Department of Hongyang Hospital between January 2014 and December 2016 were selected as pregnant women group, and at the same time, 345 non-pregnant women were selected as the nonpregnant women group. The SCL-90 test was used to analyze the mental health status of the two groups women. According to the evaluation results of SCL-90, the pregnant women were divided into mental health group (324) and psychological disorder group (143). The two groups women were surveyed by questionnaire. Logistic multivariate analysis was used to analyze the influencing factors of mental health of pregnant women.Results The scores of mental health status, obsessive-compulsive symptoms and paranoid items of non-pregnant women and pregnant women were similar. There was no statistical significance (P> 0.05). However, somatization, interpersonal relationship, depression, anxiety, hostility, fear, paranoia and psychoticism of pregnant womenwere higher than those of non-pregnant women. The difference between the two groups was statistically significant (P< 0.05). Univariate analysis and Logistic multivariate analysis showed that the number of pregnancy times 2 or more and pregnancy-free complications were the protective factors of mental health of pregnant women (OR=0.550, 0.340, P< 0.05). Unexpected pregnancy and relationships with parents-in-law and gestational age ≥ 30 weeks were risk factors of mental health of pregnant women (OR=3.296, 1.520, 2.003, P< 0.05).Conclusion Females are susceptible to mental health problems during the third trimester of pregnancy due to multiple factors such as the number of pregnancies, pregnancy intentions, family relationships, physical conditions, and gestational age. It is necessary to pay more attention on, give positive and effective psychological intervention to, and create a good pregnancy atmosphere for pregnant women.