LncRNA-HOTAIR regulates the proliferation and invasion of breast cancer cells by targeting miR-148a-3p

Objective To investigate the effects of long non-coding RNA(LncRNA) HOX transcript antisense RNA(HOTAIR) on the proliferation and invasion of breast cancer cells by targeting miR-148a-3p.

Methods T47D cell line were divided into control group, HOTAIR overexpression group and HOTAIR inhibition group through transfection experiment. Quantitative real-time polymerase chain reaction(qRT-PCR) was used to detect the expression of HOTAIR and miR-148a-3p in all 3 groups. Cell Counting Kit-8(CCK-8) and Transwell chamber method were used to detect the proliferation and invasion of transfected T47D cells. The targeted binding of HOTAIR to miR-148a-3p was detected by dual luciferase method.

Results The qRT-PCR showed that overexpression and inhibition of HOTAIR were significant(P < 0.05). Compared with the inhibition group, the expression level of miR-148a-3p in the overexpression group was significantly decreased(P < 0.05), and cell proliferation and invasion ability were significantly increased(P < 0.05). The luciferase activity of cells co-transfected with wild-type HOTAIR sequence and miR-148a-3p mimics was decreased (P < 0.05).

Conclusion LncRNA-HTOAIR may affect the proliferating and invading ability of T47D cells through miR-148a-3p.